Protective effect of anthocyanin on alcohol‐induced liver fibrosis is due to the regulation on AMPK/mTOR/autophagy pathway and energy status

Abstract

Listen

Translate article

Liver fibrosis is an advanced stage of liver damage by alcohol. We hypothesize that anthocyanin can protect alcohol induced liver fibrosis. We fed mice with high fat diet with ehanol in dringking water to induce liver fibrosis. Anthocyanin was supplied by gavage every day. We found that 12 wks feeding of high fat diet and alcohol induced lipid accumulation and increased expression of collagen α, α‐SMA, TGF‐β, and TIMP‐1 and −2, while anthocyanin supplement inhibited the increase of these parameters. In addition, we found that high fat and ethanol feeding induced increase of liver oxidative stress, increased activation of AMPK pathway, and increased autophagy, while anthocyanin supplement inhibited these effects in liver. Moreover, we found that supplement of anthocyanin reversed energy deficiency induced by high fat and ethanol feeding, including the levels of ATP, ADP, AMP, NAD+, and NMN in the liver. In conclusion, the protective effect of anthocyanin on ethanol induced liver fibrosis is due to the regulation on AMPK/mTOR/autophagy pathway, which finally regulates the energy supply in the liver.Support or Funding InformationNational Natural Science Funding (81370528, 81573142)(Yang, L), Provincial Natural Science Funding of Guangdong (2015A030313078) (Yang, L.)