Protective effect Malus pumila Mill leaf polyphenols in reserpine-induced gastric ulcer in mice

Abstract

The protective effect of polyphenols from Malus pumila leaf (MALP) on reserpine (10 mg/kg)-induced gastric ulcer in Kunming (KM) mice was investigated. Gastric juice secretion, pH of gastric juice, serum neuropeptides, including motilin (MOT), substance P (SP), vasoactive intestinal peptide (VIP), and somatostatin (SS) levels, as well as serum levels of inflammatory cytokines (TNF-α, IL-6, IL-12, and IFN-γ) were measured. Gastric occludin and p38MAPK phosphorylation was analyzed by RT-PCR or Western blotting. MALP reduced reserpine-induced gastric juice secretion and increased the pH of gastric juice. MALP increased the serum levels of VIP and SS and reduced MOT, SP, and inflammatory cytokines in serum. It also modulated the gastric expression of occludin and p38MAPK phosphorylation in mice. These results suggest that MALP showed a protective effect against reserpine-induced ulcer in mice by reducing gastric juice secretion, modulating serum neuropeptide levels, attenuating serum inflammatory cytokines, and regulating gastric levels of occludin and p38MAPK phosphorylation.