Abstract
Objective To test the protective ability of avian H9N2 virus hemagglutinin(HA)and neuraminidase(NA)DNA against lethal virus challenge in mice model.Methods Viral lung-to-lung passages in mice were used to obtain a mouse-adapted strain for avian H9N2 isolate A/chicken/Jiangsu/07/2002.DNA vaccines expressing H9N2 HA and NA,respectively,were constructed to immunize mice by electroporation.Mice were immunized once or twice(at a 3-week interval)with various dosages of DNA vaccine and were then challenged with a lethal dose(40 LD50)of mouse-adapted virus.The effectiveness of the two DNAs was evaluated by serum specific antibody titers of mice,survival rates and lung virus titers.Results Immunization once with HA or NA DNA at a dosage of 10 μg or twice at a dosage of 3 μg provided protection for mice against lethal virus challenge.Conclusion Either H9N2 virus HA or NA DNA can be effective in defending avian influenza virus infection. Key words: Avian influenza; Influenza A virus,H9N2 subtype; Vaccines,DNA; Hemagglutinins; Neuraminidase
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