Protection of bone marrow by multidrug resistance gene 1 in the liver cancer mice with intensified chemotherapy

Abstract

Objective To observe the distribution and enrichment of the foreign muhidrug resmtance gene 1(mdr1)in the bone marrow mononuclear cells and peripheral blood in the liver cancer mice with intensive chemotherapy.Methods The bone marrow mononuclear cells which transferred with retrovirus-mediated mdr1 were transplanted into Balb/c mice with liver cancer.The expression and distribution of foreign mdrl in the bone marrow mononuclear cells,peripheral blood mononuclear cells and chief organs were detected by immunohistochernistry and reverse transcriptase polymerase chain reaction(RT-PCR).And the peripheral white blood cells counting and the expression of P-glucoprotein in the tumors were monitored.Results There was no expression of mdrl in chief organs and tumors.However,the expression of mdr1 was noted in bone marrow mononuclear cells and peripheral blood mononuclear cells.The expression rates of P-glucoprotein in bone marrow mononuclear cells and peripheral blood mononuclear cells were 9.36±1.84%and 8.52±1.26%before chemotheraPY,and it was increased gradually along with intensified chemotherapy.The peripheral white blood cells countmg after chemotherapy in the transferred mdr1 gene group were significantly higher than those in the controI group(P＜0.01).Conclusions The mdr1 can be expressed in bone marrow mononuclear cells and peripheral blood mononuclear cells.The mdr1 gene therapy may provide some degrees of protection of bone marrow to increase the chemotherapy dose to kill tumor cells. Key words: Genes,MDR; Bone marrow transplantation; Gene therapy; Neoplasms