Investigation of post-radiotherapeutic hematopoietic reconstruction mediated by multidrug resistance gene 1 transfected bone marrow mononuclear cells

Abstract

Objective To investigate the post-radiotherapeutic hematopoietic reconstruction mediated by multidrug resistance gene 1 (MDR1) transfected bone marrow mononuclear cells (BM-MNCs).Methods Thirty-two BALB/c mice were randomly divided into four groups (n=8/group):normal group,blank control group,negative control group and transfection group.Mice in the blank control group,negative group and transduced group accepted 1.5Gy60Co-γ radiotherapy.After radiotherapy,normal BM-MNCs were given to mice in the blank control group;normal saline was given to mice in the negative control group by tail vein injection:MDR1 tramfected BM-MNCs were given to mice in the transduced group by tail vein injection.The following peripherals blood changes in each group were observed and determined dynamically.Results The number of the peripheral leukocytes in mice of the negative and transfeeted group increased significantly compared with that in the blank control group on the seventh day after injection.The number of the peripheral leukocytes in mice of the transfected group increased significantly compared with those in negative control group on day 7,10 and 14 after injection (2.9±0.3×109/L vs 2.7±0.2×109/L,t=2.21,P＜0.05;3.2±0.2×109/Lvs 2.8±0.2×109/L t=3.53,P＜0.05;4.2±0.3×109/L vs 3.5±0.3×109/L,t=4.73,P＜0.05).There was no statistical difference between three groups after 14 days (t=0.79,P＞0.05).Conclusions MDR1 transfected BM-MNCs can significantly increase the count of peripheral white blood cells in a short period and improve the hemopoietic microenvironment of bone marrow,thus it can promote the post-radiotherapeutic hematopoietic reconstruction. Key words: Multidrug resistance gene; Bone marrow mononuclear cells; Radiation injuries