Protection mediated by antibodies to iron-regulated outer-membrane proteins of S. typhi in a mouse peritonitis model

Abstract

Iron limitation induces the expression of iron-regulated outer-membrane proteins, which are not expressed under iron sufficient growth conditions. In the present study, these proteins were purified in order to evaluate their protective potential in the experimental model. Anti IROMPs antiserum was raised in rabbits. In mice, passively transferred anti-IROMPs antibodies provided 60% protection against the serovar Typhi challenge dose (9.6 LD50). The hyperimmune serum containing anti-IROMPs antibodies were also found to be bactericidal in the presence of complement whereas no bacterial killing was observed with pre-immunized serum. Bactericidal titre of anti-IROMPs serum was fond to be 2000 as more than 50% killing was observed with serum diluted to 1:2000. The role of IROMPs was assessed in actively-immunized mice followed by challenge with serovar Typhi. These proteins provided protection in 90% mice against challenge (480 LD50) with the pathogen. The levels of isotypes of antibodies (IgG, IgM & IgA) in the sera and secretory antibodies (sIgA) in the gut fluid of immunized mice correlated with the protection. This study, thus indicates that anti IROMPs antibodies may play an important role in providing protection at systemic as well as at mucosal level.