Abstract
Ultraviolet B (UVB) irradiation is major causative factor in skin aging. The aim of the present study was to investigate the protective effect of a 50% ethanol extract from Nypa fruticans (NF50E) against UVB-induced skin aging. The results indicated that NF50E exerted potent antioxidant activity (IC50 = 17.55 ± 1.63 and 10.78 ± 0.63 μg/mL for DPPH and ABTS-radical scavenging activity, respectively) in a dose-dependent manner. High-performance liquid chromatography revealed that pengxianencin A, protocatechuic acid, catechin, chlorogenic acid, epicatechin, and kaempferol were components of the extract. In addition, the extract exhibited elastase inhibitory activity (IC50 = 17.96 ± 0.39 μg/mL). NF50E protected against UVB-induced HaCaT cell death and strongly suppressed UVB-stimulated cellular reactive oxygen species generation without cellular toxicity. Moreover, topical application of NF50E mitigated UVB-induced photoaging lesions including skin erythema and skin thickness in BALB/C mice. NF50E treatment inhibited UVB-induced collagen degradation as well as MMP-1 and IL-1β expressions and significantly stimulated SIRT1 expression. Furthermore, the extract treatment markedly suppressed the activation of NF-κB and AP-1 (p-c-Jun) by deactivating the p38 and JNK proteins. Taken together, current data suggest that NF50E exhibits potent antioxidant potential and protection against photoaging by attenuating MMP-1 activity and collagen degradation possibly through the downregulation of MAPK/NF-κB/AP-1 signaling and SIRT1 activation.
Highlights
The skin protects against pathogens and external damage and acts as a crucial barrier between the internal and external environments of the body
Treatment with NF50E inhibited the phosphorylation of p38 and JNK (Figure 7(c)), but NF50E did not inhibit the phosphorylation of ERK1/2 (Supplementary Figure S9). These results indicate that the suppression of ultraviolet B (UVB)-stimulated p38 and JNK phosphorylation by NF50E may be required for the attenuation of Nypa fruticans (NF)-κB and activator protein 1 (AP-1) in HaCaT cells
0.0 G1 G2 G3 G4 G5 (c) sources, we found that a 50% EtOH extract of Nypa fruticans (NF50E) contained various polyphenolics, including protocatechuic acid, catechin, chlorogenic acid, epicatechin, kaempferol, and a cucurbitane triterpenoid, known as pengxianencin A (Figure 1(d))
Summary
The skin protects against pathogens and external damage and acts as a crucial barrier between the internal and external environments of the body. Exposure to chronic ultraviolet (UV) irradiation can lead to adverse pathological effects including skin damage [1]. UV-induced photoaging, which is characterized by modifications of the dermal extracellular matrix (ECM), leads to the development of wrinkles, fragility, laxity, coarseness, impaired wound healing, and increased epidermal thickness [2]. Excessive ultraviolet B (UVB) irradiation causes the generation of intracellular reactive oxygen species (ROS). This results in oxidative stress and skin inflammation through the activation of mitogenactivated protein kinase (MAPK) and upregulation of transcription factors, such as activator protein 1 (AP-1) and nuclear factor kappa B (NF-κB) [3, 4]. The development of antiaging agents that inhibit UVB-induced ROS generation is essential for suppressing the photoaging process
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