Abstract
Endogenous levels of two metabolites of prostacyclin, 6-keto-prostaglandin F 1α (spontaneous hydrolysis product) and 6,15-diketo-13,14-dihydroprostaglandin F 1α (enzymatic degradation product) were measured in urine of adults and neonates by gas chromatography-mass spectrometry, using deuterated internal standards. The method comprised extraction of prostanoids by reverse-phase cartridges and purification by silicic acid column chromatography and reverse- and straight-phase high-performance liquid chromatographies. Exogenous 6-keto-prostaglandin F 1α and 6,keto-13,14-dihydroprostaglandin F 1α added to urine were recovered quantitatively by gas chromatography-mass spectrometry. Endogenous levels of 6-keto-prostaglandin F 1α in urine of adults were 0.11 ± 0.05 (S.D.) ng/ml ( n = 12), whereas in urine of neonates the levels were much higher: 1.41±0.36 (S.D.) ng/ml ( n = 5) on the 3rd day of life declining to 0.51 ± 0.21 (S.D.) ng/ml ( n = 5) on the 5th day. 6-keto-prostaglandin F 1α was also estimated in both age groups by radioimmunoassay. Urinary levels of 6,15-diketo-13,14-dihydroprostaglandin F 1α in neonates on the 3rd day of life were 2.12 ± 0.70 (S.D.) ng/ml (n = 4) and declined until the 5th day. In adult urine this metabolite was below the limit of detection (0.20 ng/ml).
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