Abstract
Metabolism of endogenous prostacyclin was studied in adults and neonates by measuring urinary levels of 6-ketoprostaglandin F 1α (spontaneous hydrolysis product) and 2,3-dinor-6-ketoprostaglandin F 1α (enzymatically formed by β-oxidation). Quantification of prostanoids was achieved by capillary gas chromatography-mass spectrometry using the stable isotope dilution technique. Purification of the urinary lipid extract included silicic acid column chromatography and reverse- and straight-phase high-pressure liquid chromatographies. Accuracy of the method was proven by recovery experiments for both metabolites. Partial mass spectra of endogenous 6-ketoprostaglandin F 1α and 2,3-dinor-6-ketoprostaglandin F 1α were obtained from urine samples. In neonates (third day of life, n = 5 pooled urines) levels of 2,3-dinor-6-ketoprostaglandin F 1α (0.28 ± 0.18 ng/ml) were much lower than those of 6-ketoprostaglandin f 1α (2.13 ± 1.10 ng/ml), indicating low β-oxidation activity at high prostacyclin formation. In adults ( n = 7), levels of 2,3-dinor-6-ke-toprostaglandin F 1α (0.27 ± 0.21 ng/ml) and levels of 6-ketoprostaglandin F 1α (0.20 ± 0.11 ng/ml) were about the same, indicating relatively high β-oxidation at low prostacyclin formation. Values are expressed as mean ± S. D.
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