Abstract

Cell-free protein synthesis is a promising toolkit for modern biotechnology. The cell-free method has clear advantages over whole-cell systems, such as open access to the reaction and direct control over all chemical components of the mixture. The use of cell-free protein synthesis systems is promising for their use in portable diagnostics, in the expression of economically valuable proteins, in the study of cellular mechanisms and in the rationalization of protein engineering. There is a reason to expect that in the near future cell-free protein synthesis will help create
 modern biological technologies that will allow establishing the production of protein products for various purposes in a short time.

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