Abstract
Plant breeding is one of the manifestations of sustainable landscape regeneration. The CRISPR/Cas9 system is a new type of genome-directed editing technology modified from the acquired immune defense response in bacteria and archaea. Due to its efficiency and multi-function, it is widely used in animals, plants, microorganisms and other fields. In sustainable plant landscape, the system makes the gene mutate to improve plant varieties, morphological characteristics, and flowering dates, which can increase the variety of landscape. This article discusses the general situation of CRISPR/Cas9 technology and the progress of plant application, summarizing the problems and countermeasures, and looking forward to the application of sustainable landscape plants. Landscape plants are genetically complex. CRISPR/Cas9 technology can improve plants without genome references and cultivate new ornamental varieties, which can accelerate the breeding process of sustainable landscape plants.
Highlights
Plant diversity is an important manifestation of sustainable landscapes and one of the characteristics of sustainable development of the earth
CRISPR technology has high mutation efficiency which is more convenient, and it has the advantage of editing multiple genes at the same time
The mutant phenotype dicot plants obtained by CRISPR technology knockout, site-specific substitution or gene insertion and ornamental are poplar, including white poplar, petunia, chrysanthemum, clover and citrus
Summary
Plant diversity is an important manifestation of sustainable landscapes and one of the characteristics of sustainable development of the earth. The plant breeding methods of genome editing technology can mutate and recombine the genetic material of plants to obtain new plant varieties. CRISPR technology has high mutation efficiency which is more convenient, and it has the advantage of editing multiple genes at the same time It has realized the improvement of plants at the genome level and has developed into one of the most popular ways of plant gene editing. By constructing plant promoters suitable for monocotyledonous plants and dicotyledonous plants, plant mutants could be obtained and breeding of new landscape varieties will be carried out. Through the combined action of CRISPR RNA (crRNA), trans-activated crRNA and Cas endonuclease, the Cas protein complex is guided to bind to a specific PAM site to cut the DNA double-strand, which can promote the targeted cleavage of foreign DNA [2,3].Transform tracrRNA and crRNA into a sgRNA (short guide RNA) with guiding function (Figure 1). The problems of mutation efficiency, target gene specificity, targeting efficiency, and large fragment deletion were studied
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