Abstract
Objective To investigate the optimum concentration of NaClO4 treatment in order to prepare tracheal scaffold.Methods Thirty healthy New Zealand rabbits were randomly divided into 5 groups (n =6),the tracheas of group A was treated with nothing as control group,the tracheas of group B,C,D and E were treated respectively with different concentrations of NaClO4(3％,5％,6％,7％).We obtained the histologic appearance via optical microscope,the ultrastructure was observed through scanning electron microscope (SEM) and survey the structural integrity and biomechanical property of tracheal matrix.Results Histology staining showed vividly lots of epithelial cells in group A,while removed clearly in group C.SEM emerged distinctly the cilium of epithelial cells in group A.The internal surface of group B were still rough,and became smooth in group C.However the matrix in group D and E fractured more or less.Compared to the native group,the number of cell nucleus in group C were obviously decreased,the decline of DNA was statistical significance (P ＜ 0.05).There were not significantly elimination of the collagen,elastic fibers and reticular fibers in group C,as well as the contents of glycosaminoglycan (GAG) (P ＞ 0.05).We found the chondrocyte viability reserved well,but exhibited a significantly lower value of maximum tensile strength,tensile strain at break and elastic modulus with the increased concentration of NaClO4 (P ＜ 0.05).Conclusion Decellularized rabbit tracheal scaffold acquired via 5 ％ NaClO4 concentration treatment showed its structural integrity,antigen cell removal and a fine biomechanical property,which would be suitable for the application in tissue engineering tracheal allograft. Key words: Tissue engineering; Decellularization ; Tracheal scaffold ; Chondrocyte
Published Version
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