Abstract
Surfactin is one of themost powerful biosurfactants that has been known so far. It is an acidic cyclic nonribosomal lipoheptapeptide that is produced by Bacillus subtilis. In this presentation we investigated different properties of surfactin C-15. The nanomicelle forming ability of surfactin C-15 in different aqueous environments with various ionic strengths was studied by scanning electron microscope. Surfactin second structure was investigated by Far-UV CD spectrum. Its hemolytic activity and cytotoxicity were measured by hemolysis and MTT assays, respectively. Surfactin formed spherical nanomicelles in distilled water (pH = 7.4) and amorphous nanomicelles in PBS buffer (pH = 7.4). The hemolysis assay results indicated that HC50 of surfactin was 47 µM. Surfactin C-15 arrested growth of human cervix cancer HeLa cell line in a time- and dosage-dependent method, so that its IC50 at 16, 24, and 48h were 86.9, 73.1, and 50.2 µM, respectively.
Highlights
Surfactin-like lipopeptides produced by the genus Bacillus are one of the most important classes of these natural compounds
With attention to the increasing application of diverse biocompatible nanoparticles in medicine and considering that active fields of research are currently dedicated to investigate the applications of biosurfactants in pharmaceutical and biomedical sciences and knowing that the surfactin is one of the most powerful biosurfactants [10,11,12,13,14,15], in this presentation, after investigation of different properties of surfactin C-15 such as its nanomicellization ability, secondary structure, and hemolytic activity, we studied its cytotoxic effect on HeLa cell line
In this study, scanning electron microscope determined that surfactin C-15 exhibits different manners in different ionic aqueous solutions
Summary
Surfactin-like lipopeptides produced by the genus Bacillus are one of the most important classes of these natural compounds. Due to its amphiphilic structure it shows unique surface-, interface-, and membrane-active properties [3] It has hemolytic, anticoagulant, antimicrobial and antitumoral activities [1, 4,5,6] inhibition of cAMP phosphodiesterase [7], and antiHIV properties [7, 8]. Surfactins are a large variety of isoforms which differ by variation of the length and branching of their fatty acid components as well as by amino acid replacements in their peptide ring [1] How this molecule can be effective in various biological events is still largely unknown; it is speculated that the structural and lipophilic properties of surfactin may affect the stability of biological membranes [9]. With attention to the increasing application of diverse biocompatible nanoparticles in medicine and considering that active fields of research are currently dedicated to investigate the applications of biosurfactants in pharmaceutical and biomedical sciences and knowing that the surfactin is one of the most powerful biosurfactants [10,11,12,13,14,15], in this presentation, after investigation of different properties of surfactin C-15 such as its nanomicellization ability, secondary structure, and hemolytic activity, we studied its cytotoxic effect on HeLa cell line
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