Abstract
The deoxyribonucleohistone component may be physically separated from the other components of pea embryo chromatin. Such nucleohistone differs from deproteinized pea embryo DNA in its higher melting temperature, in its histone/DNA mass ratio (1·3: I) and in its inactivity in the support of DNA-dependent RNA synthesis. Pea embryo chromatin contains DNA not only as nucleohistone but also as DNA not so complexed. This is indicated by the two-step melting profile of chromatin as well as by the ability of chromatin to support DNA-dependent RNA synthesis. Approximately 80% of the DNA of pea embryo chromatin is bound in the form of nucleohistone; the remaining 20% appears to be present as DNA not complexed with histone.
Published Version
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