Abstract
We have developed a temperature controlled specimen stage on the existing jet-freezing apparatus, in order to quench samples from elevated temperatures up to 330 K. We have shown that the phase transition both from the liquid-crystalline to solid phase for pure phosphatidylcholines and from hexagonal II to lamellar phase for phosphatidylethanolamine/4% egg phosphatidic acid is prevented. Moreover, cooling rates exceeding 10,000 K s-1 were recorded indicative of ultra-rapid freezing capacity. Cryoprotectants can be omitted, since phase separation due to ice crystal growth does not occur.
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