Abstract
Specimen preparation, in particular chemical fixation, is usually the limiting factor in studies of dynamic intracellular events by electron microscopy. The main drawbacks of chemical fixation are slow mode of action (immobilization of cellular structures requires seconds to minutes) and selectivity of chemical reactivity (different molecules are inactivated at different rates). Thus, chemical fixation is much slower than many transient cellular phenomena and cannot be used to obtain reliable structural data on such events. Ultrarapid freezing can overcome these problems. The most successful ultrarapid freezing methods are "slam" and propane jet freezing.
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