Propagation of Rhinovirus on WI-38 Cell Monolayers in Rolling Bottles

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Techniques are described for the propagation of rhinoviruses on WI-38 monolayers in rolling bottles. High yields of viruses were obtained, as indicated by infectivity titers and electron microscopy. When crude harvests were subjected to low-speed centrifugation and then filtered through a 0.45-mu membrane filter, little or no loss in infectivity titer was observed. However, electron microscopic examination indicated that the concentration of viral physical particles was reduced below detectable levels after filtration. The guinea pig potency test on the lot of unfiltered rhinovirus 14 vaccine prepared in rolling bottles indicated that this vaccine stimulated higher reciprocal serum-neutralizing titers than a rhinovirus 14 vaccine prepared in stationary monolayers.