Propagation of Non-Egg-Adapted Avian Encephalomyelitis Virus in Chick Embryo Brain Cell Culture

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Non-egg-adapted avian encephalomyelitis virus (2 strains and 1 field isolate) multiplied in chick embryo brain monolayer cell cultures. The infection in the cell cultures continued persistently. Initial multiplication of the virus in the cell cultures was slow and variable among the strains and field isolate, but EID50 of about 103 per ml was obtained 14 days after inoculation. The virus activity was neutralized with specific antiserum. Both cytopathic effect and plaque did not occur. Neither the inclusion body nor AEV antigen, stained directly with the fluorescent antibody, was detected in the infected cells.