Abstract
Orchids are a very popular ornamental plants, but orchids have a very slow vegetative growth, thus requiring special treatment in the grown.To spur grow than development can be used through tissue culture techniques of multiplication. But the success of the technique of tissue culture is inseparable with give a substance on the plant growth regulator. This study aims to determine the growth an influence D.phalaenopsis Fitzg orchids of giving a gaint swith IBA administration and Kinetin according to in vitro and as well as to obtain a concentration combination of IBA and Kinetin on the best orchid D.Phalaenops is Fitzg explants. Base don’t he results of the study indicate that the combination of IBA and Kinetin concentration giving a influence percentage of live explants, number of shoots and number of roots orchid D. phalaenopsis Fitzg explants.Combination treatment of IBA 1.0 ppm and 0.1ppm Kinetin is a combination of the best treatment to produce a number of shoots orchids.The giving of IBA 0.5ppm in the medium without the addition of kinetin concentration can increase the number of roots of the orchid D.phalaenopsis Fitzg.
Highlights
Indonesia merupakan salah satu negara agraris yang memiliki potensi untuk mengembangkan berbagai tanaman hortikultura seperti tanaman hias.Salah satu jenis tanaman hias asli Indonesia yang memiliki potensi besar untuk dikembangkan secara komersial dan sebagai produk andalan adalah tanaman anggrek
This study aims to determine the growth an influence D.phalaenopsis Fitzg orchids of giving a gaint swith IBA administration and Kinetin according to in vitro and as well as to obtain a concentration combination of IBA and Kinetin on the best orchid D.Phalaenops is Fitzg explants
Terhadap Pertumbuhan Planlet AnggrekDiponegoro. Semarang. (Tidak dipublikasi). Tanaman.AgromediaPustaka. Jakarta. Tumbuhan Jilid[3].PenerbitITB. Bandung. Sodikin.2005.Pengaruh Pemberian GA3 dan K inetin terhadap Pertumbuhan kalus umbi kentang (Solanum tuberosum Var.Granola) Secarain vitro. SkripsiFMIPA Jurusan Biologi. Universitas Diponegoro. Semarang. Widiastoety D, NinaS, dan Muchtar S. 2010
Summary
Bahan yang digunakan dalam penelitian ini adalah eksplan anggrek Dendrobium phalaenopsis Fitzg yang ditanam tanpa hormon, stok zat pengatur tumbuh IBA (Indole-3-butyricacid) dan Kinetin, media Vacint and Went (VW). Rancangan Acak Lengkap (RAL) dengan pola factorial yang terdiri dari dua factor meliputi konsentrasi IBA (faktor pertama) dengan konsentrasi 0ppm, 0,5ppm, 1,0 ppm dan 1,5 ppm. Dan Kinetin (faktor kedua) dengan konsentrasi 0 ppm, 0,1 ppm, 0,5 ppm dan 1,0 ppm. Kombinasi kedua faktor tersebut menghasilkan 16 perlakuan , setiap perlakuan dilakukan ulangan 3 kali. Parameter pertumbuhan yang diamati adalah persentase hidup eksplan, jumlah tunas dan jumlah akar. Data yang diperoleh kemudian dianalisis menggunakan ANOVA dengan uji lanjut DMRT (Duncan Multiple Range Test) pada taraf 5%
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