Abstract
Some species of Brazilian orchids are practically extinct in their natural habitats. Large scale propagation of in vitro native orchids is a way of preserving them. Specific culture media for some species have already been described, but there are no studies on Catasetum fimbriatum and Cyrtopodium paranaensis. The aim of the present study was to establish in vitro propagation protocol of C. fimbriatum and C. paranaensis to reintroduce these species in natural habitat. The traditional culture media were: MS; MS modified with ½ macronutrients; MS modified with ¼ macronutrients, Vacin & Went and Knudson C. The commercial fertilizers formulations were: N.P.K (10-5-5) 2 mL L-1 and formulation N.P.K (10-30-20) 3 g L-1. The data, assessed six months after the beginning of the experiment, consisted of: number of roots, main root length, pseudobulb diameter, plant dry weight, root dry weight and plant height. The best results of C. fimbriatum plant development were obtained using MS medium complete and MS medium modified with half of macronutrients for canopy length. For the number of roots, the best results were obtained using MS modified with half of macronutrients. The best results for C. paranaensis plant development were obtained using MS modified with half of the macronutrients for canopy length and MS modified with ¼ of macronutrients for number of roots.
Highlights
The Orchidaceae family is one of the largest of the Angiosperm families, consisting of about 700 genera and 35,000 species distributed throughout the world
The aim of the present study was to establish in vitro propagation protocol of C. fimbriatum and C. paranaensis to reintroduce these species in natural habitat
The two Brazilian orchid species used in this study were Catasetum fimbriatum (Morren) Lindl. and Cyrtopodium paranaensis Schlt
Summary
The Orchidaceae family is one of the largest of the Angiosperm families, consisting of about 700 genera and 35,000 species distributed throughout the world. Orchids are found in all the states of Brazil, and in greater quantity in the Mata Atlantica Forest), which goes from Pernambuco state to Rio Grande do Sul. Forest), which goes from Pernambuco state to Rio Grande do Sul These plants are found in abundance in riverside forest throughout the country, in the Savannah of Central Brazil, in the Amazon forest and even in the Northeastern tropical thorn bush forest (caatinga). There are more than 50 species of Catasetum in the world and a great variety of hybrids, where the flower can vary greatly in size and color. It is a tropical plant and generally easy to grow (Hersh, 1996). The Cyrtopodium paranaensis orchid is a tropical climate species typical of Brazilian hillsides. The Cyrtopodium genus is found in the Americas from Florida (USA) to Argentina (Meneses, 1995a, b)
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