Abstract
Biomaterial-associated infections are a problem in the use of endoprosthetic materials in the palliative treatment of malignant obstructive jaundice. Fibronectin has been reported to mediate adherence of bacteria to host tissue and biomaterials. Adsorption of fibronectin to rubber--representing material used for biliary drainage--and subsequent adherence of Escherichia coli strain PSS1 and E. coli strain NG7C (which binds to immobilised fibronectin) were investigated. Quantitative adsorption of fibronectin to rubber slices was studied with 125I-labelled, purified human plasma fibronectin. In buffer solutions, fibronectin showed a high affinity for rubber slices. Adherence of the E. coli strains to uncoated rubber slices was similar and was significantly inhibited by the presence of plasma components and bile. Adherence of E. coli PSS1 to fibronectin-coated slices was poor. In contrast, E. coli NG7C adhered efficiently to coated slices in proportion to the amount of adsorbed fibronectin; adherence was not reduced by the presence of albumin or bile, or the fibronectin-binding ligands gelatin, heparin and fibrinogen. However, pre-digestion of coated slices with trypsin significantly reduced adherence.
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