Prolonged follicular dominance is associated with dysregulated proteomic profile of the follicular fluid in Bos indicus cows

Abstract

Prolonged follicular dominance is one of the conditions associated with disconcerted follicular dynamics that result in substantial economic losses to the farmers through low reproductive efficiency in cattle. Hormonal aberrations associated with prolonged follicular dominance may affect the follicular microenvironment and composition of follicular fluid. The current study focused on proteome changes of follicular fluid in prolonged follicular dominance compared to physiological follicular dominance. Prolonged dominance was induced in Deoni cows (n = 6) by using CIDR (previously used for 7 days) from day 4–8 of estrus, with PGF2 injection on day 6 and day 7 at 12 h intervals. Follicular fluid was collected by ultrasound guided transvaginal follicular aspiration method. Global proteomic analysis of follicular fluid revealed 217 proteins in the Deoni cow, with the majority of proteins involved in 21 pathways, 42 molecular functions, and 106 biological processes. Complement and coagulation cascades (22.8%) and cholesterol metabolism (4.68%) were the major pathways in which identified proteins were involved. Comparison of physiological and prolonged dominant follicular fluid revealed differential expression of 26 proteins, of which 15 were upregulated and 11 were downregulated. Proteins involved in complement and coagulation cascades, and vitamin digestion and absorption were found to be dysregulated in PFD. The present study suggests that the expression of proteins involved in inflammation, oocyte metabolism, and ovulation cascade were found to be dysregulated in the follicular fluid of prolonged follicular dominance consequently resulting in delayed ovulation or anovulation.