Abstract
Summary Application of water stress (10% w/v polyethylene glycol 6000, PEG) to potato cell suspension cultures resulted in an increase of the proline pool from 2.3 to 20.7 μmol/g dry mass. Despite this proline accumulation in stressed potato cells, no in vitro changes in relative activities of key enzymes of basic metabolic pathways thought to be involved in proline biosynthesis could be detected. The pyridine nucleotide reduction charges, which are measures of the nucleotide signaling system of a cell, were also the same in stressed and non-stressed cells. The results are discussed in terms of water-stress-induced changes of the physico-chemical state of the aqueous cytoplasm. These changes of the microenvironment of the aqueous cytoplasm probably alter the efficiency of the multi-enzyme reaction chains involved in proline biosynthesis.
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