Abstract
Summary ABA, GA and KN treatments on proline metabolism during senescence were studied in leaf disks of Horsegram ( Macrotyloma uniflorum Lam.) incubated in the dark. Senescence, being accelerated by ABA treatments and retarded by GA and KN treatments, is evidenced from the relative depletion in chlorophyll and protein, and an increase in free amino acids and proline. The enzymes involved in oxidation of proline, proline dehydrogenase and proline oxidase showed no change; the enzymes involved in supplying NADPH for proline biosynthesis, glucose-6-phosphate dehydrogenase and 6-phospho gluconate dehydrogenase were inhibited, suggesting that the accumulated proline in senescing leaf disks of horsegram is due to loss of protein budget.
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