Abstract
Overexpression of MSN2, which is the transcription factor gene in response to stress, is well-known to increase the tolerance of the yeast Saccharomyces cerevisiae cells to a wide variety of environmental stresses. Recent studies have found that the Msn2 is a feasible potential mediator of proline homeostasis in yeast. This result is based on the finding that overexpression of the MSN2 gene exacerbates the cytotoxicity of yeast to various amino acid analogs whose uptake is increased by the active amino acid permeases localized on the plasma membrane as a result of a dysfunctional deubiquitination process. Increased understanding of the cellular responses induced by the Msn2-mediated proline incorporation will provide better comprehension of how cells respond to and counteract to different kinds of stimuli and will also contribute to the breeding of industrial yeast strains with increased productivity.
Highlights
Cells of microorganisms are frequently exposed to a variety of stresses in their environment, such as prolonged nutrient starvation, free radicals and toxic molecules, imbalances in osmotic pressure and pH level, and non-optimal growth temperatures (Mager and De Kruijff, 1995; Ruis and Schüller, 1995)
Our study found that quadruple disruption of proline permease genes (GAP1, PUT4, AGP1, and GNP1) in MSN2OE cells with the strain CAY29 background conferred higher resistance to AZC (Mat Nanyan et al, 2019a), similar to that observed in wild-type cells with the same quadruple disruption (Andréasson et al, 2004)
In this mini-review, we discuss the current understanding of the stress-responsive transcription factor Msn2 and the stress response in yeast
Summary
Cells of microorganisms are frequently exposed to a variety of stresses in their environment, such as prolonged nutrient starvation, free radicals and toxic molecules, imbalances in osmotic pressure and pH level, and non-optimal growth temperatures (Mager and De Kruijff, 1995; Ruis and Schüller, 1995). Glutamine plays a substantial role in mammalian cell growth where it facilitates the transport of other amino acids such as leucine into the cells and subsequently activates mTORC1 (Gonzalez and Hall, 2017) These multiple regulatory mechanisms at the transcriptional, protein, and metabolic levels constitute a network that protects yeast cells from harmful conditions and allows them to adapt to new environments. Once yeast cells are challenged by environmental perturbations, Msn2/4 are rapidly dephosphorylated and translocated into the nucleus (Görner et al, 1998; Beck and Hall, 1999) They bind to the stress-response element sequence (STRE; AGGGG) in the promoter region of the target genes and subsequently activate the transcription (Boy-Marcotte et al, 1998, 1999; Gasch et al, 2000; Causton et al, 2001). Our recent study showed that the overexpression of MSN2 shortens the replicative lifespan of yeast cells by reducing the intracellular proline levels (Mukai et al, 2019)
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call