Abstract
Present study was conducted to assess the regeneration potential and producing mass-clonal seedlings of Rose through shoot tip culture. A total of 40 explants were cultured on each of basal medium supplemented with different concentrations of BAP, NAA and GA. The observations on the survival rate, days taken to initiate the shoots, total number of shoots and length of shoots, initiation of roots, total number of root, length of roots and number of leaves were investigated. Rose regenerated on MS-Basal medium (control) without addition of growth hormones showed significantly (P < 0.05) lower survival rate and did not show shoots up to the end of experiments. MS-Basal medium supplemented with different concentrations of BAP, NAA and GA showed increasing rate of survival. MS-Basal medium supplemented with highest BAP, NAA and GA concentration (MS-SV) has taken least time to initiate the shoots, whilst supplemented in concentrations of 0.5 and/or 1.0 mg/L, respectively revealed more time. Rooted plants were transplanted into the substrate and acclimatized in the laboratory greenhouse (humid cavity). The acclimatization in the humid cavity showed optimistic effect on the number of survived plants.
Highlights
Rose (Rosa hybrid L.) is one of the most important and economic ornamental plants worldwide [1]
It was observed that rose explants cultured on MS medium without addition of hormones (MS-SI) showed significantly (P < 0.05) lower survival rate (72.50%) compared to explants on MS medium supplemented with various concentrations of BAP, NAA and GA
It is of a interest to note that there were no any significant differences (P > 0.05) found to be amongst the survival rate of rose explants cultured on MS medium supplemented with concentrations of 0.5 mg/L, 1.0 mg/L or 2.0 mg/L each of BAP, NAA and GA i.e. 75.5 0 ± 0.28, 75.47 ± 0.18 and 75.33% ± 0.8%, respectively
Summary
Rose (Rosa hybrid L.) is one of the most important and economic ornamental plants worldwide [1]. In vitro multiplication of rose is very useful because it provides a means of overcoming difficulties of producing large numbers of relatively homogenous seedlings and system was extensively used in wide range of tissue culture investigations carried-out by many scientists. The findings of these techniques provided an argument for the use of tissue and cell culture techniques as tools for rose improvement [2]. Production of a sexual embryos and their subsequent development into free-living plants was the first published report in the literature has obtained free-living plants from clonal propagated explants tissues derived from shoot tips, lateral buds and inflorescence. Similar studies were begun in various Institutes of the world [4]
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