Effect of Different Concentrations of Kinetin and NAA on Micropropagation of Gardenia Jasminoides

Abstract

The goal of this study was to use plant tissue culture technique in vegetative propagation of
 Gardenia (Gardenia jasminoides) by using single nodes and shoot tips excised from soft cuttings and
 treated with different concentrations of growth regulators.The results revealed that the use of different
 disinfistants was highly effective in reducing cultures contamination. The use of mercuric chloride (0.1%,
 HgCl2) for 10 minutes was very effective in preventing contamination and gave the highest survival
 percentage (99%). During the culture of shoot tips at initiation stage, a nutrient medium contained (2
 mgl-1 kinetin+ 0.6 mgl-1 NAA) gave the highest values of average number of shoots and leaves and growth
 length (2.3 shoots/ explant, 2.4 leaves and 1.9 cm respectively).At vegetative multiplication stage, The
 culture of shoot tips on a nutrient medium containing (3 mgl-1 kinetin) gave the highest values of
 average number of shoots and leaves (2.9 shoots/ explant, 3.6 leaves respectively).As for growth length,
 the results revealed that the highest value was achieved by MS medium supplemented by 2 mgl-1 kinetin +
 0.1 mgl-1 NAA (3.3 cm). Regarding rooting process, MS medium supplemented by 4 mgl-1 NAA gave the
 highest average number and length of roots (4.0 roots and 2.50 cm respectively).Also, the medium
 supplemented by 8 mgl-1 IAA gave the highest number and length of roots (3.40 roots and 3.50 cm
 respectively). Plantlets obtained were transferred to pots and acclimatized with 95 % success.