Abstract
Background. Spoil heaps chronosequences are convenient models to analyze the succession of microbiome during restoration of anthropogenically disturbed landscapes. The investigation of the heavy metal content in lands with mining activity, can be used as an indicator of ecosystem recovery.
 Materials and methods. Objects were technozems of 1-year, 25- and 50-year-old embryonic soils, and control soil under forest. Quantitative polymerase chain reaction (qPCR) and NGS-sequencing of V4 region of 16S rRNA gene were applied. Results. During the soil-forming process, an increase organic carbon and nitrogen, as well as a gradual increase archaeal 16S rRNA gene copies and in the number of Bradyrhizobiaceae, Blastocatellaceae, Xantobacteriaceae. Although we found a number of taxa that increased during soil-forming process (Thaumarchaeota, Bradyrhizobiaceae, Blastocatellaceae, Xantobacteriaceae), technozems of different ages had a similar structure and diversity of prokaryotic communities, differing from a nature soil. Biodiversity analysis revealed that technozems generally had a similar structure and diversity of prokaryotic communities, significantly differing from the mature soil a specific clusterization of microbiomes. The HM contents and bacterial abundances remained at the same level in chronosequence.
 Conclusions. The 50 years of soil development on overburden spoil heaps is not enough for the recovery from HM contamination and restoration of soil ecosystem functioning.
Highlights
Spoil heaps chronosequences are convenient models to analyze the succession of microbiome during restoration of anthropogenically disturbed landscapes
We found a number of taxa that increased during soil-forming process (Thaumarchaeota, Bradyrhizobiaceae, Blastocatellaceae, Xantobacteriaceae), technozems of different ages had a similar structure and diversity of prokaryotic communities, differing from a nature soil
Biodiversity analysis revealed that technozems generally had a similar structure and diversity of prokaryotic communities, significantly differing from the mature soil a specific clusterization of microbiomes
Summary
❀ Проанализированы физико-химические параметры, растительное сообщество и структура прокариотных комплексов микробиомов однолетних (с растительным покровом и без него), 25- и 50-летних эмбриональных почв (техноземов), сформированных в районе Курской магнитной аномалии (КМА, Россия). Полученные результаты показывают, что пятидесяти лет недостаточно для развития почвы на отвалах вскрышных пород, установления в ней экологически безопасного уровня тяжелых металлов и восстановления функционирования почвенной экосистемы. We found a number of taxa that increased during soil-forming process (Thaumarchaeota, Bradyrhizobiaceae, Blastocatellaceae, Xantobacteriaceae), technozems of different ages had a similar structure and diversity of prokaryotic communities, differing from a nature soil. Экогенез на отвалах вскрышных пород в районах добычи часто характеризуется высоким содержанием тяжелых металлов (ТМ), влияющим на сукцессию микроорганизмов во время почвообразования/почвовосстановления. Целью данного исследования является анализ временной динамики содержания тяжелых металлов и структуры и численности прокариотических сообществ в молодых почвах (техноземах), сформировавшихся в районе КМА, на разных этапах почвообразования (1, 25, 50 лет). Таблица 1 Описание растительности техноземов, сформированных на отвалах лёссовидных суглинков Курской магнитной аномалии
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
