Abstract
Normal programmed multiple development (PMD) causes longitudinal spot reconcentration, but does not counter lateral diffusion. If the periodic evaporation of solven characteristic of PMD is caused to occur preferentially along the center line of the developing chromatogram, solven from each side flows with a component towar that first-dyring center line. This flow produces lateral spot reconcentration. Normal PMD complemented by lateral spot reconcentration is call centered PMD. An easily reproducible and usable mask produes centered PMD, which is demonstrated on ordinary precoated plates. Grooves in the thin-layer bed can also produce centered PMD. A centered-PMD spot produced on an ordinary pre-coated plate has minimized, round are 1–3 mm in diameter. With a given plate, this area reflects the whole of a given molecular population. Such a spot does not spread with longer developments, but merely separates further from its neighbors.
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