Abstract
Aim. To determine the prognostic significance PCA3 gene expression in urine sediment and exosomes in patients with localized prostate cancer (PC) and associated histologic changes in the peritumoral zone as a predictor of biochemical recurrence after radical prostatectomy (RPE).
 Materials and methods. Of 148 patients with localized PC, 96 (65%) had high-grade prostatic intraepithelial neoplasia (PIN-2) in the peritumoral zone. The other 52 (35%) had no pathologic tissue of the peritumoral zone. PDA3 expression in urine sediment and exosomes was determined with real-time PCR with respect to the reference gene KLK3.
 Results. The PCA3 gene expression level in urine exosomes in patients with PIN-2 in the prostatic peritumoral zone and synchronous pancreatic adenocarcinoma was higher among patients with subsequent disease recurrence. Increased PCA3 gene expression in the urine sediment was also predictive of the risk of recurrence of a prostatic tumor with PIN-2 in the peritumoral zone, although to a lesser degree than the results with urine exosomes. When the ∆Ct РСА3-KLK3 was ≥1,86 in the urine sediment, biochemical recurrence of PC and PIN-2 developed more frequently in the peritumoral zone (84% versus 51%, p = 0,013).
 Conclusions. Increased PCA3 gene expression in urine sediment and exosomes is a predictor of increased risk of biochemical recurrence after RPE in patients with localized PC and PIN-2 in the peritumoral zone.
Highlights
Цель — определить прогностическую значимость оценки экспрессии гена РСА3 в осадке и экзосомах мочи у больных с локализованным раком предстательной железы (РПЖ) и сочетанными гистоморфологическими изменениями в перитуморальной зоне при определении риска биохимического рецидива после радикальной простатэктомии (РПЭ)
Of 148 patients with localized prostate cancer (PC), 96 (65%) had highgrade prostatic intraepithelial neoplasia (PIN-2) in the peritumoral zone
The PCA3 gene expression level in urine exosomes in patients with PIN-2 in the prostatic peritumoral zone and synchronous pancreatic adenocarcinoma was higher among patients with subsequent disease recurrence
Summary
ISSN 2225-9074 оригинальные СТАТЬи to the reference gene KLK3. Results. The PCA3 gene expression level in urine exosomes in patients with PIN-2 in the prostatic peritumoral zone and synchronous pancreatic adenocarcinoma was higher among patients with subsequent disease recurrence. Increased PCA3 gene expression in the urine sediment was predictive of the risk of recurrence of a prostatic tumor with PIN-2 in the peritumoral zone, to a lesser degree than the results with urine exosomes. When the ∆Ct РСА3-KLK3 was ≥1,86 in the urine sediment, biochemical recurrence of PC and PIN-2 developed more frequently in the peritumoral zone (84% versus 51%, p = 0,013). Increased PCA3 gene expression in urine sediment and exosomes is a predictor of increased risk of biochemical recurrence after RPE in patients with localized PC and PIN-2 in the peritumoral zone. Целью работы было определение прогностической значимости оценки экспрессии гена РСА3 в осадке и экзосомах мочи у больных с локализованным РПЖ и сочетанными гистоморфологическими изменениями в перитуморальной зоне при определении риска биохимического рецидива (БР) после радикальной простатэктомии (РПЭ)
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