Abstract

Long noncoding RNA (LncRNA) is closely associated with the development of colorectal cancer (CRC). The chip data and clinical information of GSE104364 and GSE151021 were downloaded by GEOquery. Limma and Kaplan–Meier analysis were performed. Lnc-S100B-2 was obtained, and high expression of Lnc-S100B-2 was predicted to be associated with a lower survival rate. Online software was adopted to predict downstream regulatory genes, and miR-331-3p and Mixed Lineage Leukemia Translocated to 10 (MLLT10) were screened and verified. After silencing Lnc-S100B-2 and MLLT10, the proliferative activity of CRC cells decreased, and the apoptosis rate increased. At the gene and protein levels, the expressions of PCNA, Ki67, and Bcl-2 were decreased in the sh-Lnc-S100B-2 group, sh-MLLT10 group, and sh-Lnc-S100B-2 + sh-MLLT10 group, while the expressions of cleaved caspase 3, caspase 9, and Bax were increased. In vivo, the volume and mass of the tumor decreased in the sh-Lnc-S100B-2 + sh-MLLT10 group. Proliferation and apoptosis-related index (PCNA, Ki67, cleaved caspase 3, caspase 9, Bax, and Bcl-2) expression level was also altered. Meanwhile, the infiltration of immune cells (CD3 (-), CD16 (+), and CD11b (+) cells) decreased. The expressions of epithelial-mesenchymal transformation (EMT) related indicators (E-cadherin, N-cadherin, Vimentin, β-catenin, Snail, and Slug) were changed. E-cadherin and β-catenin were increased in the sh-Lnc-S100B-2 + sh-MLLT10 group, while N-cadherin, vimentin, snail, and slug were decreased. In conclusion, our study found that the expression of Lnc-S100B-2 was dysregulated in CRC. Lnc-S100B-2 could affect cell apoptosis and the microenvironment of CRC through regulating MLLT10.

Highlights

  • Colorectal cancer (CRC) is one of the most common malignant tumors in humans and the fourth deadliest cancer in the world, with nearly 900,000 deaths every year [1]

  • Lnc-S100B-2 Is Highly Expressed with a Poor Prognosis in CRC

  • We found a series of differentially expressed Long noncoding RNA (LncRNA) in CRC (Figure 1(a)

Read more

Summary

Introduction

Colorectal cancer (CRC) is one of the most common malignant tumors in humans and the fourth deadliest cancer in the world, with nearly 900,000 deaths every year [1]. CRC has become a major global public health problem [2]. Studies have shown that some progress has been made in diagnosing, treating, and preventing CRC. E poor prognosis of CRC patients remains a major problem [6]. CRC patients are usually diagnosed as advanced, with a poor prognosis and a low 5-year survival rate [7]. Previous studies have shown that the poor prognosis of CRC is related to molecular and gene changes [8]. Differential genes and molecules have essential research value in CRC [9,10]

Objectives
Methods
Results
Conclusion
Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call