Effects of long intergenic non-coding RNA 00152 on proliferation and apoptosis of colorectal cancer cells

Abstract

Objective To study the expression of long intergenic non-coding RNA 00152 (Linc00152) in colorectal cancer (CRC) and cell lines. To investigate the effect of Linc00152 on proliferation and apoptosis of CRC cells. Methods The expression levels of Linc00152 in 31 CRC tissues and adjacent normal tissues, four CRC cell lines (CCL244, SW480, HT29, LoVo) were detected by real-time quantitative polymerase chain reaction(Real-time PCR). Linc00152 was knockdown in CCL244 and HT29 by small interfering RNA (siRNA). Methyl thiazolyl tetrazolium (MTT) assay and clone formation assay were conducted to evaluated the cell proliferation, flow cytometry was used to evaluated the apoptosis. Results Real-time PCR showed that the expression of Linc00152 in CRC tissues (4.18±3.43) was 27.07 times higher than that in the adjacent normal tissues (8.94±3.07, P=0.045). Linc00152 was highly expressed in CRC cell lines. Linc00152 was efficiently down-regulated by siRNA. Inhibition of Linc00152 in CCL244 cells impaired the colony-forming capacity [(26.67±0.47)% vs. (44.67±1.89)%, P=0.006]. MTT assay revealed that the cell viability of CCL244 and HT29 was inhibited in siRNA group [72 h: CCL244 (23.48±3.49)%, HT29 (12.53±2.86)%, P=0.001]. Down-regulation of Linc00152 in CCL244 and HT29 cells induced cells apoptosis [CCL244 (9.08±0.22)% vs. (4.15±0.29)% (P=0.003), HT29 (16.36±0.70)% vs. (7.11±0.88)%, P=0.007]. Conclusion The expression of Linc00152 was high in CRC. Loss of Linc00152 can inhibit cell proliferation and promote cell apoptosis in CRC cells. Key words: Colorectal cancer; Long non-coding RNA; Long non-coding RNA 00152; Proliferation; Apoptosis