Prognostic and immunological role of Fam20C in pan-cancer

Lixue Liu,Shouli Guo,Wenxia Xu,Ying Li,Yuanbo Zhan,Zhongshuang Liu,Xiaoyao Liu,Junlong Da,Han Jin,Yawei Geng,Xinpeng Liu,Xinjian Zhang,Bin Zhang,Jianqun Wang

doi:10.1042/bsr20201920

Abstract

Background: The family with sequence similarity 20-member C (Fam20C) kinase plays important roles in physiopathological process and is responsible for majority of the secreted phosphoproteome, including substrates associated with tumor cell migration. However, it remains unclear whether Fam20C plays a role in cancers. Here, we aimed to analyze the expression and prognostic value of Fam20C in pan-cancer and to gain insights into the association between Fam20C and immune infiltration.Methods: We analyzed Fam20C expression patterns and the associations between Fam20C expression levels and prognosis in pan-cancer via the ONCOMINE, TIMER (Tumor Immune Estimation Resource), PrognoScan, GEPIA (Gene Expression Profiling Interactive Analysis), and Kaplan–Meier Plotter databases. After that, GEPIA and TIMER databases were applied to investigate the relations between Fam20C expression and immune infiltration across different cancer types, especially BLCA (bladder urothelial carcinoma), LGG (brain lower grade glioma), and STAD (stomach adenocarcinoma).Results: Compared with adjacent normal tissues, Fam20C was widely expressed across many cancers. In general, Fam20C showed a detrimental role in pan-cancer, it was positively associated with poor survival of BLCA, LGG, and STAD patients. Specifically, based on TCGA (The Cancer Genome Atlas) database, a high expression level of Fam20C was associated with worse prognostic value in stages T2–T4 and stages N0–N2 in the cohort of STAD patients. Moreover, Fam20C expression had positive associations with immune infiltration, including CD4+ T cells, macrophages, neutrophils, and dendritic cells, and other diverse immune cells in BLCA, LGG, and STAD.Conclusion: Fam20C may serve as a promising prognostic biomarker in pan-cancer and has positive associations with immune infiltrates.

Highlights

Protein kinases are a common way of regulating information transduction in organisms, which play a crucial role in the process of cell signal by transferring a phosphate from adenosine triphosphate (ATP) to the target proteins [1,2]
In a trial conducted on the bioinformatics and human lung adenocarcinoma (LUAD) cells, hypoxia was indicated a poor prognostic factor for LUAD, and family with sequence similarity 20-member C (Fam20C) was identified as a key gene associated with hypoxia in the progression of LUAD [24]
We found that the expression of Fam20C was negatively correlated with tumor purity of LUAD, and positively correlated with immune cells infiltration, which further verified the relationship between Fam20C expression and poor prognosis (Supplementary Figure S3)

Summary

Introduction

Protein kinases are a common way of regulating information transduction in organisms, which play a crucial role in the process of cell signal by transferring a phosphate from adenosine triphosphate (ATP) to the target proteins [1,2]. The family with sequence similarity 20-member C (Fam20C) kinase plays important roles in physiopathological process and is responsible for majority of the secreted phosphoproteome, including substrates associated with tumor cell migration. It remains unclear whether Fam20C plays a role in cancers. Conclusion: Fam20C may serve as a promising prognostic biomarker in pan-cancer and has positive associations with immune infiltrates

Objectives

Methods

Results

Conclusion