Abstract
OBJECTIVESEvidence suggests that symptoms of TMJD vary over menstrual cycle indicating that changes in steroid sex hormone modulate TMJ pain. The objective of the study is to determine modulatory role of P4 on cytokine/chemokine expression in TMJ.METHODSSprague Dawley ovariectomized female rats were divided in 2 groups with 1 group receiving progesterone pellets & subcutaneous P4 injections to recapitulate physiological plasma levels of progesterone. Groups were subdivided further & were given bilateral TMJ injections of Complete Freund's adjuvant (CFA) or saline within the superior joint space of TMJ during diestrous‐2 and proestrous phase. So 4 groups include P4/diestrous‐2/saline, P4/diestrous‐2/CFA, P4/proestrous/saline & P4/proestrous/CFA.Rats were sacrificed 24 hrs later. The cytokine/chemokine expression was analyzed in retrodiscal tissue, trigeminal ganglia & subnucleus caudalis using arrays.RESULTSThe transcript expression of cytokines IL‐6 and IL‐1<â > decreased by 50 & 82 fold &chemokines CCL3, CCL20, CXCL1, CXCL2 and CXCL3 decreased by 103, 91, 86, 118 and 155 fold, comparing the P4/diestrous‐2/saline group to P4/proestrous/saline group suggesting P4 modulates expression of these molecules in TMJ.CONCLUSIONThe data suggest that cyclical plasma levels of P4 in females can modulate cytokines/chemokines expression in TMJ. Supported by NIDCR DE016059‐01 (LLB) & DE15372 (PRK).
Published Version
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