Abstract
BackgroundPorcine reproductive and respiratory syndrome virus (PRRSV) is an enveloped virus, bearing severe economic consequences to the swine industry worldwide. Previous studies on enveloped viruses have shown that many incorporated cellular proteins associated with the virion's membranes that might play important roles in viral infectivity. In this study, we sought to proteomically profile the cellular proteins incorporated into or associated with the virions of a highly virulent PRRSV strain GDBY1, and to provide foundation for further investigations on the roles of incorporated/associated cellular proteins on PRRSV's infectivity.ResultsIn our experiment, sixty one cellular proteins were identified in highly purified PRRSV virions by two-dimensional gel electrophoresis coupled with mass spectrometric approaches. The identified cellular proteins could be grouped into eight functional categories including cytoskeletal proteins, chaperones, macromolecular biosynthesis proteins, metabolism-associated proteins, calcium-dependent membrane-binding proteins and other functional proteins. Among the identified proteins, four have not yet been reported in other studied envelope viruses, namely, guanine nucleotide-binding proteins, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase, peroxiredoxin 1 and galectin-1 protein. The presence of five selected cellular proteins (i.e., β-actin, Tubulin, Annexin A2, heat shock protein Hsp27, and calcium binding proteins S100) in the highly purified PRRSV virions was validated by Western blot and immunogold labeling assays.ConclusionsTaken together, the present study has demonstrated the incorporation of cellular proteins in PRRSV virions, which provides valuable information for the further investigations for the effects of individual cellular proteins on the viral replication, assembly, and pathogenesis.
Highlights
Porcine reproductive and respiratory syndrome virus (PRRSV) is an enveloped virus, bearing severe economic consequences to the swine industry worldwide
Extensive proteomic analysis has been performed on human cytomegalovirus (HCMV) virions, human immunodeficiency virus (HIV), emiliania huxleyi virus 86 (EhV-86) virions, kaposi’s sarcoma-associated herpesvirus (KSHV) and influenza virus, that shows the presence of lots of cellular proteins [17,18,19,20,21]
Purification of PRRSV virions Marc-145 cells were infected with a PRRSV strain i.e., GDBY1, isolated from dead pig[30]. 96 h post infection, the supernatant was harvested and concentrated through a 20% (w/v) sucrose cushion prepared in TNE buffer (Tris-buffered saline including 50 mM Tris, 100 mM NaCl, 1 mM EDTA, pH 7.4)
Summary
Porcine reproductive and respiratory syndrome virus (PRRSV) is an enveloped virus, bearing severe economic consequences to the swine industry worldwide. Previous studies on enveloped viruses have shown that many incorporated cellular proteins associated with the virion’s membranes that might play important roles in viral infectivity. Numerous host proteins have been identified that incorporate into the membranes or inside the envelopes of the virions during their budding from the host cells, but the role and importance of these host cellular proteins in virus infection are not fully understood [14,15,16]. Tubulin, heat shock protein (Hsp) 90 and Hsp were detected in Epstein-Barr virus (EBV) [28], while b-actin was identified to interact with infectious bronchitis virus M protein, subsequently confirms to play important roles in virion assembly and budding [29]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
