PROFILE OF SEX STEROIDS AT EARLY STAGES OF LIVER METASTASIS FROM SARCOMA 45 IN MALE RATS

Abstract

Background . Sex hormones are involved in pathogenesis of cancer of the reproductive organs; however, their role in the development of other cancers is poorly studied. Objectives. Study of metabolism of sex hormones in the testes, prostate, tumor (TR), metastases (MTS) and blood serum (BS) at the early stages of liver metastasis. Material and methods. The study included 28 white out bred male rats. Levels of estradiol (E 2 ), total testosterone (T), prolactin (PRL) and progesterone (P 4 ) were studied in the liver by radioimmunoassay, and free testosterone (fT), estrone ( Е 1 ) and sex steroid-binding globulin(SSBG)were studied by ELISA 1-2 weeks (before TR occurrence in the spleen) and 5 weeks (before MTS occurrence in the liver) after the injection of TR cells into the spleen. Results. Appearance of TR in the spleen was preceded by increased Е 1 (by 3.4 times) and fT (by 2.5 times) and decreased P 4 (by 4.5 times) in week 1 and increased PRL (by 1.7 times) and decreased fT (by 3.4 times) in week 2. Already formed TR showed E 1 increase (by 8.8 times). Occurrence of MTS was preceded by increased E 2 (by 1.2 times) and decreased P 4 (by 3.1 times) in the liver. Conclusions. E isthe main element of the hormonal profile of primary TR. Е 1 creates conditions for the formation of the metastatic field in the liver where later MTS develop.