Profile of fatty acids, vitamins, phytosterols and phenolic acids in Trachystemon orientalis L. and evaluation of their antioxidant activity

Abstract

The present study focuses on the phytochemical content and antiradical properties of Trachystemon orientalis L., which grows spontaneously and is consumed as food in rural areas of Düzce (from Turkey) province. DPPH, ABTS and OH radical scavenging tests were utilized for identifying the antiradical properties of the extracts of this plant. In addition, the metal chelating potential of this plant was also evaluated. The higher the inhibition % value calculated in these tests, the higher the antioxidant activity was considered and the results were evaluated. T. orientalis the average ABTS radical cleaning test results of extracts prepared in different concentrations of methanol, ethanol and purified water were found to be 93.35%, 91.32%, 94.70%, respectively. The average DPPH radical cleaning test results of the extracts of this plant prepared in different concentrations of methanol, ethanol and purified water were determined as 37.26%, 7.95%, 52.26%, respectively. T. orientalis the OH radical cleaning test results of the extracts of plant prepared from methanol, ethanol and purified water were determined as 60.85%, 61.33%, 24.12%, respectively. In addition, the metal chelation test results of methanol, ethanol and pure water extracts of this plant were found to be 63.57%, 42.04%, 82.64%. It was determined that the highest content of protein (20.93 mgBSA/g), proanthocyanidin (13.76 µg CE/g) and phenolic (3621.03 µg GAE/g) was found in T. orientalis pure water extract. It has been determined that gallic acid (187.67 μg/g), vanillic acid (3.78 μg/g), rosmarinic acid (10.55 μg/g) and hydrocynamic acid (0.78 μg/g) are found in different proportions in T. orientalis plant. It has been observed that T. orientalis is an important source of palmitic acid, stearic acid, oleic acid, linoleic acid, γ-linolenic acid, alpha-linolenic acid, gadoleic acid and docosahexaenoic acid. It has been established that this plant has a low content of fat-soluble vitamins and phytosterols.