Proferrorosamines and phytopathogenicity in Erwinia spp.

Abstract

Proferrorosamine A (pFR A) of the plant pathogenic bacterium Erwinia rhapontici was shown to inhibit growth of wheat and cress seedlings at the > or = 10 ppm level. When the seeds were continuously exposed to 100 ppm pFR A, the germination of cress and wheat seeds was inhibited up to 90% and 80%, respectively. The inhibition could be reversed through addition of equimolar amounts of ferrous iron, which indicates that the strong iron chelating capability of pFR A is responsible for the observed effect. The Fe(II) in the corresponding iron complex, ferrorosamine A, was found to be remarkably resistant towards oxidation by hydrogen peroxide and therefore redox-cycling in the Haber-Weiss cycle. It is thus conceivable that pFR A may also attenuate the generation of reactive hydroxyl radicals during the resistant and would reaction. The apparent correlation between proferrorosamine production and virulence in erwiniae was further corroborated through the analysis of Erwinia persicinus, a newly described species. Using electrospray ionization mass spectrometry, E. persicinus was shown to produce pFR A and pFR B, and preliminary evidence for the phytopathogenicity of E. persicinus was found in cress. Inhibition of wheat seedlings by E. persicinus could not be demonstrated, but this may be due to technical difficulties or different host specificities. Taken together, our results indicate that the phytopathogenicity of E. rhapontici and E. persicinus may, as least in part, be due to the release of proferrorosamines.