Abstract
This study aimed to determine the levels of seric tumor necrosis factor-alpha (TNF-alpha) and production of hydrogen peroxide (H2O2) by peritoneal macrophages in mice experimentally infected with T. cruzi and submitted to pre-infection exercise training and to post-infection acute exercise. Female 30-day-old BALB/c mice were inoculated with 1,400 blood trypomastigotes of Y-strain T. cruzi. Exercise programs consisted in moderate-intensity activity and were carried out in a treadmill. The measurements were performed with material collected at the 13th day after infection Serum TNF-alpha was evaluated using capture ELISA. H2O2 production was expressed by coloration produced after incubation of peritoneal macrophages and the measurement was performed using an ELISA reader. There were no statistically significant differences in TNF-alpha levels and H2O2 production between the trained and non-trained infected groups. Thus, the physical training performed before infection and physical exercise performed after the infection were not able to change the levels of TNF-alpha and production of H2O2 in the infection by T. cruzi.
Highlights
O Trypanosoma cruzi é o agente etiológico da doença de Chagas que afeta entre 16 e 18 milhões de pessoas na América Latina (MONCAYO, 2003)
O programa de treinamento físico foi realizado em esteira rolante durante oito semanas, com uma sessão diária de treinamento, cinco vezes na semana, com duração de 30 a 45 min. na primeira semana, 45 a 60 min. na segunda semana e 60 min. nas demais
A maior concentração de TNF-alfa, tanto no grupo submetido ao treinamento físico pré-infecção quanto no grupo infectado e subsequentemente submetido ao exercício físico, quando comparados com seus respectivos controles infectados pode ser um indício de influência do exercício físico na resposta à infecção pelo T. cruzi
Summary
O Trypanosoma cruzi é o agente etiológico da doença de Chagas que afeta entre 16 e 18 milhões de pessoas na América Latina (MONCAYO, 2003). Para a produção de H2O2, macrófagos peritoneais de três animais de cada grupo, obtidos no 13o dia de infecção, foram lavados com PBS a 1.500 rpm por 10 min.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
