2-DEOXYGLUCOSE PROMOTES ANTI-INFLAMMATORY POLARIZATION OF PERITONEAL MACROPHAGES IN MICE WITH LEWIS LUNG CARCINOMA

Abstract

Summary. Aim: to investigate the effect of 2-deoxyglucose at a wide range of concentrations on the polarization of peritoneal macrophages of intact mice and mice with Lewis lung carcinoma. Object and methods: peritoneal macrophages obtained from intact female C57BL/6 mice and Lewis lung carcinoma-bearing mice. isolation of peritoneal macrophages, determination of nitric oxide production and arginase activity. Results: 2-deoxyglucose does not affect nitric oxide production and arginase activity of peritoneal macrophages of intact mice. 2-deoxyglucose at a concentration of 10 mM significantly (by 17%, p<0.05) increases arginase activity in peritoneal macrophages of mice with Lewis lung carcinoma. Conclusions: It was revealed that peritoneal macrophages in mice with Lewis lung carcinoma have a pro-inflammatory M1 phenotype. The addition of 2-deoxyglucose at a concentration of 10 mM to the incubation medium of peritoneal macrophages obtained from mice with Lewis lung carcinoma promotes a switch in macrophage polarization to the M2 phenotype.