Abstract
Abstract Four sterile diploid interspecific hybrids were obtained by pollinating precociously exserted stigmas of Ornithopus pinnatus with pollen from O. sativus while buds were still closed, and culturing the resulting hybrid embryos in vitro with nurse endosperm. Doubling the chromosome number of one hybrid by treatment of shoot tips with colchicine gave partial restoration offertility. In vitro embryo culture was necessary to raise naturally selfed F2, F1, and F4 generations as endosperm development was impaired. However, occasional F4 seeds showed almost normal endosperm development, suggesting that in situ maturation of seeds may be achieved in future generations. The project was aimed at combining the high productivity of O. sativus with the prostrate habit, hard-seededness, and foliar tannin production of O. pinnatus. F1 and F4 individuals derived from a single tetraploid F2 plant showed a combination of the leafiness of O. sativus with a trailing habit more like that of O. pinnatus and variation i...
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