Abstract
The galacto-oligosaccharides and Saccharomyces boulardii are very useful for the intestinal microbiota. The yeast acts as probiotic and used for the reduction of monosaccharides from GOS mixture. The present study was concerned with the production of synbiotic product from GOS and S. boulardii through transgalactosylation process. The GOS was produced from β-galactosidase (156 U/1 ml of lactose solution) of Kluyveromyces lactis using lactose (250g/L) as substrate at 37ºC. The GOS mixture was analyzed through thin layer chromatography and megazyme kit. The maximum production of GOS occurs after 5 hr at 37ºC with phosphate buffer (pH 6.5). The GOS mixture was treated with probiotic S. boulardii. The monosaccharides were reduced at 37ºC after 4 hrs using 300µl yeast/5 ml GOS mixture. Then synbiotic product was formed and undergoes lyophilization procedure. The total yeast count was 3.9 × 104CFU/g in lyophilized product. The in vitro bifidogenic effect was determined. Bifidobacterium shows more positive effect towards prebiotic and combined effect of prebiotic and probiotic as compared to Lactobacillus.
Highlights
The synergistic combination of prebiotic and probiotic is known as synbiotic
At equal concentration of lactose solution and enzyme concentration (1:1), there is no production of galactooligosaccharides (GOS)
The prebiotics enhance the growth of probiotic bacteria in the synbiotic product and helpful for host’s microflora (Underwood et al, 2009)
Summary
The synergistic combination of prebiotic and probiotic is known as synbiotic. The prebiotic favors the probiotic and activates the host’s beneficial intestinal microflora (Underwood et al, 2009). GOS are carbohydrates that are non-digestible and fermented by colon bacteria They have great role in functional foods due to their benefits such as mineral absorption, role in lipid breakdown and anti-inflammatory (Schaafsma, 2008), show less potential to dental caries. GOS can be produced using lactose through transgalactosylation process with the help of β-galactosidase enzyme. The mono and disaccharides are produced that are not required in prebiotic production They are undesirable because they increase the caloric value of product by absorbing in small intestine. Saccharomyces bulardii is well known yeast which often used as probiotic and dietary supplement It reduces the diarrhea, alleviates inflammatory diseases related to intestine, helpful in prevention of infection of Helicobacter pylori, inhibits the growth of pathogens (Geyik et al, 2006) and stimulates antibody production against diarrhea causing toxin (Qamar et al, 2001). There is a need to produce synbiotic at commercial level in Pakistan due to its beneficial effects and increasing demands
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