Abstract
Biosynthesis of polyhydroxyalkanoate (PHA) from raw cassava starch as the carbon source by bacteria was investigated in this study. About 300 bacterial colonies were isolated from soil samples. Among them, sixteen bacterial strains were found to produce PHA from cassava starch. Strain D8 produced the highest poly(3-hydroxybutyrate) (PHB) content of 69.8 wt% was selected for further studies. Strain D8 was classified under the Bacillus megaterium group based on 16S rRNA gene sequences. High cell dry weight (CDW) of 6.5 g\L and poly(3- hydroxybutyrate) (PHB) content of 66.2 wt% were obtained by strain D8 after 21 h of cultivation in a bioreactor using batch culture mode. In this study, Bacillus megaterium D8 exhibited high promise for reducing the production cost of PHB.
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