Abstract
Bioconversion of food waste oil (FWO) into biodegradable plastic is a promising method for converting waste into high-value products. In this study, a strain (Pseudomonas sp. H3) was isolated for polyhydroxyalkanoate (PHA) synthesis from FWO. After 72h of cultivation with 20g/L of FWO, the high cell dry weight (CDW) of 3.6g/L, PHA yield of 2.4g/L, and PHA content of 65wt% were obtained under the optimal temperature (25°C) and inoculum amount (6% (v/v)). Fed-batch fermentation was conducted in a 5 L bioreactor with a maximum CDW of 16g/L, PHA content of 54wt%, and PHA productivity of 0.23g/(L·h) after 36h. The PHA had a molecular weight of 54 782Da and a low polydispersity index of 1.41 with glass transition, melting, and degradation temperatures of -20°C, 34°C, and 210°C, respectively. To further utilize the wastewater after PHA production, anaerobic digestion was employed for CH4 production, and the CH4 yield was 284mL/g volatile solids. Microbial community analysis showed that the abundance of acetate-oxidizing bacteria and Methanobacterium significantly increased during anaerobic digestion. This study describes a new strain for the economical synthesis of biodegradable plastics and presents a novel framework for fully utilizing FWO with the production of PHA and CH4.
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