Abstract
The present investigation was focused on the production of extracellular β-mannanase from newly isolated Penicillium aculeatum APS1 using palm kernel cake and soyabean meal which are the residual by-products of oil extraction industry, under solid state fermentation. On supplementing palm kernel cake with 20% soyabean meal, yield of β-mannanase production was reached to 2807 U/g. Response surface methodology was used for statistical optimization of β-mannanase production. Two independent variables, namely moisture level and incubation time, were found to be significantly contributing for the production process. Under optimized condition of moisture level (52.25%) and incubation time (130 h), the yield of β-mannanase was improved by 1.6 fold and maximum activity of β-mannanase was 4696 U/g. The optimum temperature and pH for crude β-mannanase were 65 °C and 6.0, respectively. Crude and partially purified β-mannanase was found to be effective in release of mannooligosachharides by hydrolysis of mannan rich substrates viz. locust bean gum, guar gum and konjac glucomannan. Qualitative and quantitative analysis of MOS was carried out by TLC and ion chromatography. β-Mannanase from Penicillium aculeatum APS1 was found to have properties suitable for applications in feed/food industry.
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