Abstract
ABSTRACTThe results obtained from this work strongly indicate that the solid state fermentation (SSF) system using the palm kernel cake (PKC) as a substrate is an economical method for the production of β-mannanase at extremely low operational cost based on the fact that PKC is one of the cheap and abundant agro-waste by-products of the palm oil industry. Under initial conditions, i.e. 2 mm particle size of PKC, the moisture ratio of 1:1 of PKC:moistening agent and pH 7, Malbranchea cinnamomea NFCCI 3724 produced 109 U/gram distribution of the substrate (gds). The production of β-mannanase was optimised by the statistical approach response surface methodology (RSM) using independent variables, namely initial moisture (12.5), pH (9.0) and solka floc (100 mg). Noticeably, six fold enhancement of β-mannanase production (599 U/gds) was obtained under statistically optimised conditions. HPLC results revealed that β-mannanase is an endo-active enzyme that generated manno-oligosaccharides with a degree of polymerisation (DP) of 3 and 4. Semi-native PAGE analysis revealed that M. cinnamomea produced three isoforms of mannanase. Selective production of oligosaccharide makes M. cinnamomea β-mannanase an attractive enzyme for use in food and nutraceutical industries.
Highlights
The lignocellulosic biomass is composed of lignin, cellulose and hemicellulose
The results obtained from this work strongly indicate that the solid state fermentation (SSF) system using the palm kernel cake (PKC) as a substrate is an economical method for the production of β-mannanase at extremely low operational cost based on the fact that PKC is one of the cheap and abundant agro-waste by-products of the palm oil industry
Only a few thermostable β-mannanases have been characterised from these (Puchart et al 2004; Yang et al 2015). Keeping this point in mind we evaluated a thermophilic fungus, M. cinnamomea NFCCI 3724 for optimised production of β-mannanase on PKC using the response surface methodology approach
Summary
The lignocellulosic biomass is composed of lignin, cellulose and hemicellulose. From the industrial point of view, economical and efficient conversion of these polymers, especially cellulose and hemicellulose, to their constituent monomer is very important (Moreira and Filho 2008). Hemicellulose is the second most abundant heteropolymer composed of mainly pentoses (xylose, arabinose), hexoses (mannose, glucose, galactose) and sugar acids (Chauhan et al 2012). Mannan is the second major hemicellulose after xylan that occurs as a hemicellulose fraction of soft and hard wood. Endo-1, 4-β-Dmannanase (EC 3.2.1.78) is a hemicellulolytic enzyme that randomly hydrolyses 1,4-β-D-mannopyranosyl linkages within the main chains of mannans and heteropolysaccharides such as galactomannan, glucomannan and galactoglucomannan. Other accessory enzymes involved in mannan breakdown include β-mannosidase, β-glucosidase and α-galactosidase that liberate mannose, glucose and galactose from mannan
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
