Abstract
Summary A local strain of Kluyveromyces lactis with high potentiality for lactase biosynthesis was isolated from acid whey. The yeast propagated on permeate medium produced the highest yield of lactase activity, 16,900 lactose units per liter of medium. The yeast grew on a medium containing up to 5% sodium chloride concentration, with a decrease in the net yields of lactase activity. Toluene autolysis proved to be slightly better than benzene in extracting lactase from sodium chloride containing media. The presence of magnesium and manganese in the enzymatic assay mixture had a significant effect on the hydrolysis rate of lactase extracted from yeast grown on media containing sodium chloride. The results obtained lend themselves to feasible industrial applications.
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