Abstract
The present study was conducted with an aim to scale up the production of iturin A using soybean curd residue (okara). Iturin A was produced by indigenous bacterial strain Bacillus subtilis RB14-CS through glass column reactor (GCR) under solid state fermentation (SSF) was characterized. The enhanced iturin A production was observed with respect to enhanced substrate bed height when SSF was conducted in Erlenmeyer flask. To check the effect of substrate bed height on iturin A production under SSF of okara, GCR was introduced. Substrate bed height of 15 cm was suitable for iturin A production which was about 2700 mg/kg wet substrate. The observed iturin A production by the aerobic bacteria Bacillus subtilis in nearly anaerobic condition in such high substrate bed for SSF is a wonderful finding for development of SSF system in future.
Highlights
The extensive human activities in agriculture and food industries produce a substantial amount of waste
Iturin A was produced by indigenous bacterial strain Bacillus subtilis RB14-CS through glass column reactor (GCR) under solid state fermentation (SSF) was characterized
B. subtilis RB14-CS is an aerobic bacterium, it was thought that the increased substrate bed height might create more anaerobic condition into substrate bed and production of iturin A would be reduced
Summary
The extensive human activities in agriculture and food industries produce a substantial amount of waste. These wastes have become no longer negligible to the atmosphere. The only solution for the decent waste management is the recycling of waste. Agriculture and food industries wastes, for exam-. Ple, wheat straw, rice hulls, corn cobs and okara are being recycled as solid substrates for SSF [1,2,3]. This process requires relatively low energy whereas produces higher concentrated products. Feeds, fuel, enzymes and antibiotics have been produced through SSF [1,2]
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