Abstract
Sauerkraut brine was found to be a favorable substrate for the production of diacetyl reductase (EC 1.1.1.5) by Geotrichum candidum ATCC 34614. The mold yielded more diacetyl reductase (nearly 0·8 unit/mg protein) than other microorganisms reported previously. The enzyme had an optimum pH of 6·0 and showed no loss of activity in the pH range of 6·0 to 9·0. The enzyme was stable up to 40°C, but lost 40% of its activity at 50°C in 30 min. The K m value for diacetyl was calculated to be 0·107 m M.
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