Abstract
Colubrina glandulosa has potential for reforestation of disturbed areas. Seedlings inoculated with arbuscular mycorrhizal fungi (AMF) can be produced with biosolids from Sewage Treatment Plants. This study aimed to evaluate the response of Colubrina glandulosa seedlings to isolated or mixed inoculation of different AMF species on a biosolid-based substrate under greenhouse conditions. The experimental design was completely randomized, with 32 replicates (seedlings)/treatment and five treatments: (1) control or absence of inoculation (CT); (2) inoculation with Dentiscutata heterogama (DH); (3) inoculation with Gigaspora margarita (GM); (4) inoculation with Rhizophagus clarus (RC); (5) inoculation with the mixture of the three AMF species (MT). We evaluated height and collar diameter of the seedlings at 30, 60, 90, 120, and 140 days after the experiment installation. We evaluated shoot dry biomass (SDM), root dry biomass (RDM), and total dry biomass; SDM / RDM ratio; Dickson Quality Index; relative increments in height, collar diameter, and shoot biomass; nutrient content (N, P, K) in the shoots and roots; efficiency of absorption and utilization of nutrients; rate of root mycorrhizal colonization, at 140 days. In general, the highest values of growth and nutrition variables occurred in the MT treatment, which was recommended, in comparison with CT, DH, GM, and RC. Colonization rates were low (≤ 20 %), regardless of the mycorrhizal inoculum, due to the high levels of phosphorus in the biosolid.
Highlights
Colubrina glandulosa Perkins belongs to the Rhamnaceae family
Studies should be carried out focusing on the production of seedlings with higher quality, for the use in forest recovery programs. This context includes investigations about the inoculation of seedlings with arbuscular mycorrhizal fungi (AMF), which show higher quality when compared to non-inoculated seedlings (SCABORA et al, 2010; OLIVEIRA JÚNIOR et al, 2017)
Each container was filled with approximately 1.0 kg of substrate consisting of a mixture of 20 % vermiculite and 80 % sewage sludge biosolid, which came from the Alegria Sewage Treatment Plant (STP), located in Rio de Janeiro, RJ
Summary
Colubrina glandulosa Perkins (popularly known as “saguaraji-vermelho” or “sobrasil”) belongs to the Rhamnaceae family. This initial secondary tree species has rapid growth and can be planted in full sun, which promotes the formation of a microclimate favorable to the growth and establishment of forest species of more advanced successional groups, which require greater shading (SILVA et al, 2015). Studies should be carried out focusing on the production of seedlings with higher quality, for the use in forest recovery programs. This context includes investigations about the inoculation of seedlings with arbuscular mycorrhizal fungi (AMF), which show higher quality when compared to non-inoculated seedlings (SCABORA et al, 2010; OLIVEIRA JÚNIOR et al, 2017)
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